The US has said it regrets Bolivia’s decision to move against USAID programmes in the country.
President Evo Morales had accused the organisation to conspire against Bolivia.
US state department spokesman Patrick Ventrell denied the accusations.
"We think the programmes have been positive for the Bolivian people, and fully co-ordinated with the Bolivian government and appropriate agencies under their own national development plan," he said.
USAID said it regretted the decision in a statement.
"Those who will be most hurt by the Bolivian government's decision are the Bolivian citizens who have benefited from our collaborative work on education, agriculture, health, alternative development, and the environment," the statement said.
Bolivian President expels USAID (01 May 2013)